Selectivity Agent

Worried about cell selectivity, expression, and stability of your mRNA?

We've got you covered. We don't guess — we optimize, while respecting the biology. Every nucleotide and its position matters.

Evaluate your sequence →

mCherry · CD4+ T-cell lead

Target expression (CD4+)6×
Off-target (Hepatocyte)↓ low
Off-target (B-cell Mutu)↓ low
Objectives optimized7
The Challenge

mRNA design is a balancing act.

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Strengthening structural stability can reduce targeting precision - losing cell selectivity.
Strengthening structural stability can reduce targeting precision - losing
cell selectivity.
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Product Strengthening structural stability can reduce targeting precision - losing cell selectivity.
Product Strengthening structural stability can reduce targeting precision -
losing cell selectivity.
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Tuning for cell selectivity frequently trades off protein yield - hurting expression.
Tuning for cell selectivity frequently trades off protein yield — hurting expression.

These tradeoffs are not bugs — they are biology.

Selectivity Agent

Worried about
cell selectivity, expression, and stability
Of your mRNA?

We've got you covered.We don't guess. We Optimize, while respecting the biology!
Every nucleotide and its position matters

Solution

Every nucleotide optimized across different objectives at once.

5′ UTR (BPE) CDS (3-mer codon) 3′ UTR (BPE) [MASK]
Use Case

Cell Selectivity — Target high. Off-target suppressed.

mCherry mRNA optimized for CD4+ T-cells. All 5 top leads achieve 6× selectivity across both off-target cell types.

Top 5 Leads — Protein Expression

Consistently high in target. Low everywhere else.

Protein expression across three cell types. Leads reach 5–6× selectivity.

CD4+ (target) Hepatocyte B-cell Mutu

Population-Level Improvement

MCSOA designs: high in target, low in off-target.

Each point is one sequence. Optimized (teal) vs original (blue-gray).

opt. (Mutu,CD4) opt. (Hep,CD4) orig. (Mutu,CD4) orig. (Hep,CD4) 1:1 line

X: Protein Expression in Bcells_Mutu / Liver_Hepatocyte · Y: Protein Expression in Tcells–CD4. Optimized points sit above the 1:1 line — high in target, low off-target.

Training Convergence

Cell Selectivity

 Target (Primary CD4)  off target 1 (Hepatocyte)  off target 2 (Mutu)
Ready to check your design?
Evaluate your Sequence →